class b cpg oligonucleotide odn2006 Search Results


class b cpg odn 2006 cpg b  (Miltenyi Biotec)
93
Miltenyi Biotec class b cpg odn 2006 cpg b
Class B Cpg Odn 2006 Cpg B, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+b+cpg+oligonucleotide+odn2006/ODN+2006/pmc07332672-23-12-30
Average 93 stars, based on 1 article reviews
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class b cpg oligonucleotide odn2006  (InvivoGen)
97
InvivoGen class b cpg oligonucleotide odn2006
Inhibition of TLR2‐dependent NF‐κB activation and IL‐8 secretion. A) HEK‐Blue hTLR2 cells were pre‐incubated with CU‐CPT22 or compounds 1 – 6 and 9 – 11 for 1 h and then stimulated additionally with TLR2/1 agonist Pam 3 CSK 4 (10 ng/mL) or TLR2/6 agonist Pam 2 CSK 4 (1 ng/mL) for 24 h. B) HEK‐Blue hTLR2 cells were pre‐incubated with increasing concentrations of 4 , 6 , 9 , 10 and then stimulated additionally with Pam 3 CSK 4 or Pam 2 CSK 4 for 24 h. Supernatants were tested for TLR2‐mediated NF‐κB/AP‐1 activity by quantification of SEAP release (OD 640 ). C) THP‐1 macrophages were incubated with CU‐CPT22 (25 μM) or compound 6 for 1 h and afterwards stimulated with Pam 3 CSK 4 (10 ng/mL), Pam 2 CSK 4 (1ng/mL), LPS (10 ng/mL), flagellin (1 μg/mL), CL075 (8 μM) or <t>ODN2006</t> (5 μM) for 24 h. Supernatants were analyzed for IL‐8 secretion by ELISA. Mean+SD or ±SD ( n =3).
Class B Cpg Oligonucleotide Odn2006, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+b+cpg+oligonucleotide+odn2006/ODN+2006/pmc07496872-177-32-40
Average 97 stars, based on 1 article reviews
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class b cpg oligodeoxynucleotides odn-2006  (Midland Certified Reagent)
90
Midland Certified Reagent class b cpg oligodeoxynucleotides odn-2006
Inhibition of TLR2‐dependent NF‐κB activation and IL‐8 secretion. A) HEK‐Blue hTLR2 cells were pre‐incubated with CU‐CPT22 or compounds 1 – 6 and 9 – 11 for 1 h and then stimulated additionally with TLR2/1 agonist Pam 3 CSK 4 (10 ng/mL) or TLR2/6 agonist Pam 2 CSK 4 (1 ng/mL) for 24 h. B) HEK‐Blue hTLR2 cells were pre‐incubated with increasing concentrations of 4 , 6 , 9 , 10 and then stimulated additionally with Pam 3 CSK 4 or Pam 2 CSK 4 for 24 h. Supernatants were tested for TLR2‐mediated NF‐κB/AP‐1 activity by quantification of SEAP release (OD 640 ). C) THP‐1 macrophages were incubated with CU‐CPT22 (25 μM) or compound 6 for 1 h and afterwards stimulated with Pam 3 CSK 4 (10 ng/mL), Pam 2 CSK 4 (1ng/mL), LPS (10 ng/mL), flagellin (1 μg/mL), CL075 (8 μM) or <t>ODN2006</t> (5 μM) for 24 h. Supernatants were analyzed for IL‐8 secretion by ELISA. Mean+SD or ±SD ( n =3).
Class B Cpg Oligodeoxynucleotides Odn 2006, supplied by Midland Certified Reagent, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+b+cpg+oligonucleotide+odn2006/class+b+cpg+oligodeoxynucleotides+odn+2006/pm17785797-52-2-23
Average 90 stars, based on 1 article reviews
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class b cpg odn 2006  (LabForce AG)
90
LabForce AG class b cpg odn 2006
Inhibition of TLR2‐dependent NF‐κB activation and IL‐8 secretion. A) HEK‐Blue hTLR2 cells were pre‐incubated with CU‐CPT22 or compounds 1 – 6 and 9 – 11 for 1 h and then stimulated additionally with TLR2/1 agonist Pam 3 CSK 4 (10 ng/mL) or TLR2/6 agonist Pam 2 CSK 4 (1 ng/mL) for 24 h. B) HEK‐Blue hTLR2 cells were pre‐incubated with increasing concentrations of 4 , 6 , 9 , 10 and then stimulated additionally with Pam 3 CSK 4 or Pam 2 CSK 4 for 24 h. Supernatants were tested for TLR2‐mediated NF‐κB/AP‐1 activity by quantification of SEAP release (OD 640 ). C) THP‐1 macrophages were incubated with CU‐CPT22 (25 μM) or compound 6 for 1 h and afterwards stimulated with Pam 3 CSK 4 (10 ng/mL), Pam 2 CSK 4 (1ng/mL), LPS (10 ng/mL), flagellin (1 μg/mL), CL075 (8 μM) or <t>ODN2006</t> (5 μM) for 24 h. Supernatants were analyzed for IL‐8 secretion by ELISA. Mean+SD or ±SD ( n =3).
Class B Cpg Odn 2006, supplied by LabForce AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+b+cpg+oligonucleotide+odn2006/class+b+cpg+odn+2006/pmc12174074-98-23-28
Average 90 stars, based on 1 article reviews
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nuclease resistant phosphorothioate odn2006  (TIB MOLBIOL)
90
TIB MOLBIOL nuclease resistant phosphorothioate odn2006
Inhibition of TLR2‐dependent NF‐κB activation and IL‐8 secretion. A) HEK‐Blue hTLR2 cells were pre‐incubated with CU‐CPT22 or compounds 1 – 6 and 9 – 11 for 1 h and then stimulated additionally with TLR2/1 agonist Pam 3 CSK 4 (10 ng/mL) or TLR2/6 agonist Pam 2 CSK 4 (1 ng/mL) for 24 h. B) HEK‐Blue hTLR2 cells were pre‐incubated with increasing concentrations of 4 , 6 , 9 , 10 and then stimulated additionally with Pam 3 CSK 4 or Pam 2 CSK 4 for 24 h. Supernatants were tested for TLR2‐mediated NF‐κB/AP‐1 activity by quantification of SEAP release (OD 640 ). C) THP‐1 macrophages were incubated with CU‐CPT22 (25 μM) or compound 6 for 1 h and afterwards stimulated with Pam 3 CSK 4 (10 ng/mL), Pam 2 CSK 4 (1ng/mL), LPS (10 ng/mL), flagellin (1 μg/mL), CL075 (8 μM) or <t>ODN2006</t> (5 μM) for 24 h. Supernatants were analyzed for IL‐8 secretion by ELISA. Mean+SD or ±SD ( n =3).
Nuclease Resistant Phosphorothioate Odn2006, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/class+b+cpg+oligonucleotide+odn2006/cpg+odn+1826/pm18977183-49-20-30
Average 90 stars, based on 1 article reviews
nuclease resistant phosphorothioate odn2006 - by Bioz Stars, 2026-11
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Image Search Results


Inhibition of TLR2‐dependent NF‐κB activation and IL‐8 secretion. A) HEK‐Blue hTLR2 cells were pre‐incubated with CU‐CPT22 or compounds 1 – 6 and 9 – 11 for 1 h and then stimulated additionally with TLR2/1 agonist Pam 3 CSK 4 (10 ng/mL) or TLR2/6 agonist Pam 2 CSK 4 (1 ng/mL) for 24 h. B) HEK‐Blue hTLR2 cells were pre‐incubated with increasing concentrations of 4 , 6 , 9 , 10 and then stimulated additionally with Pam 3 CSK 4 or Pam 2 CSK 4 for 24 h. Supernatants were tested for TLR2‐mediated NF‐κB/AP‐1 activity by quantification of SEAP release (OD 640 ). C) THP‐1 macrophages were incubated with CU‐CPT22 (25 μM) or compound 6 for 1 h and afterwards stimulated with Pam 3 CSK 4 (10 ng/mL), Pam 2 CSK 4 (1ng/mL), LPS (10 ng/mL), flagellin (1 μg/mL), CL075 (8 μM) or ODN2006 (5 μM) for 24 h. Supernatants were analyzed for IL‐8 secretion by ELISA. Mean+SD or ±SD ( n =3).

Journal: Chemmedchem

Article Title: Biological Characterization, Mechanistic Investigation and Structure‐Activity Relationships of Chemically Stable TLR2 Antagonists

doi: 10.1002/cmdc.202000060

Figure Lengend Snippet: Inhibition of TLR2‐dependent NF‐κB activation and IL‐8 secretion. A) HEK‐Blue hTLR2 cells were pre‐incubated with CU‐CPT22 or compounds 1 – 6 and 9 – 11 for 1 h and then stimulated additionally with TLR2/1 agonist Pam 3 CSK 4 (10 ng/mL) or TLR2/6 agonist Pam 2 CSK 4 (1 ng/mL) for 24 h. B) HEK‐Blue hTLR2 cells were pre‐incubated with increasing concentrations of 4 , 6 , 9 , 10 and then stimulated additionally with Pam 3 CSK 4 or Pam 2 CSK 4 for 24 h. Supernatants were tested for TLR2‐mediated NF‐κB/AP‐1 activity by quantification of SEAP release (OD 640 ). C) THP‐1 macrophages were incubated with CU‐CPT22 (25 μM) or compound 6 for 1 h and afterwards stimulated with Pam 3 CSK 4 (10 ng/mL), Pam 2 CSK 4 (1ng/mL), LPS (10 ng/mL), flagellin (1 μg/mL), CL075 (8 μM) or ODN2006 (5 μM) for 24 h. Supernatants were analyzed for IL‐8 secretion by ELISA. Mean+SD or ±SD ( n =3).

Article Snippet: TLR2 ligands : The synthetic lipopeptides Pam 3 CSK 4 and Pam 2 CSK 4 , lipopolysaccharide from Escherichia coli O111:B4 (LPS‐EB), flagellin from Salmonella typhimurium (FLA‐ST), the thiazoloquinoline compound CL075 and class B CpG oligonucleotide ODN2006 were purchased from Invivogen and the TLR2 antagonist CU‐CPT22 was obtained from Sigma‐Aldrich.

Techniques: Inhibition, Activation Assay, Incubation, Activity Assay, Enzyme-linked Immunosorbent Assay